Technical Resources
Technical Resources – Sample Preparation
Preparation of Minimal Salts Media
Growth Procedure for H2O Minimal Salts Media
Growth Procedure for D2O Minimal Salts Media
Buffer Exchange Procedure for the Preparation of NMR Samples
Effect of pH on the NMR Spectra
Technical Resources – Operating the NMR Spectrometers & Data Acquisition and Processing
Step-by-step procedure for NMR data acquisition
Chemical shift referencing and temperature calibration
How to process a 2D HSQC spectrum
How to measure and analyze 15N T2 relaxation times.
Assigning Proteins Using Sparky
NMRPipe Reference Page
Bruker Manuals
Pulse program manual
Processing manual
Acquisition manual