Optical Imaging Facility Equipment

Please consult with facility staff prior to anticipated usage.

Confocal / Multiphoton Microscopy
System 1 Zeiss LSM710
Laser Scanning Confocal Microscopy
Room STRF 252

 

Objectives: Lasers:
  • 10X 0.3 NA (Plan-Neofluoar)
  • 20X 0.8 NA (Plan-Apo Chromat)
  • 40X NA 1.3 Oil DIC (Plan-Neofluoar)
  • 60X NA 1.4 Oil DIC (Plan-Apochromat)
  • 100X NA 1.4 Oil (Plan-Apochromat)
  • Blue Diode (405 nm)
  • Argon (458, 488, and 515nm)
  • Laser diode 561 nm
  • HeNe 594 nm
  • HeNe 633 nm

 

Axioimager Z1 upright microscope channel spectral channels, DIC, motorized stage, physiological software, 3D image analysis.

 

System 2 Olympus FV-1000
Laser Scanning Confocal Microscopy
Room 2.518U Olympus IX-81 Microscope

 

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
 Olympus FV1000 Laser SOP

 

Objectives: Lasers:
  • 10X 0.40 NA (UPlanSApo, DIC)
  • 20X 0.75 NA (UPlanApo, DIC)
  • 25X 1.05 NA (XLPlan N, Water)-
    Available upon special request
  • 40X 0.60 NA (LUCPlanFLN, DIC)
  • 40X 1.30 NA (UPlanFLN, Oil, DIC)
  • 40X 1.00 NA (UPlanApoN, Oil, DIC)
  • 60X 1.42 NA (PlanApoN, Oil, DIC)
  • 60X 1.10 NA (LUMFI, Water) – Available upon request
  • Blue Diode (405nm)
  • Argon (458nm, 488nm, 515nm)
  • Green HeNe (543nm)
  • Red Diode (635nm)
  • Chameleon Multiphoton tunable laser, 715nm – 900nm

 

Spectral scanning
3 Channel Detection for Fluorescence
Brightfield Detection of DIC images
Z-stepper motor
Fluoview and Tiempo Ratio Imaging Software
LSM Technology Objective Inverter
Non-descanned external detector

 

 

System 3 Zeiss 510 NLO
(Confocal and Multiphoton)
Room 2.518U Zeiss Axiovert 200 Microscope

 

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
 Zeiss Laser SOP

 

Objectives: Lasers:
  • 10X 0.30 NA (Plan-NEOFLUAR)
  • 40X 1.30 NA (Plan-NEOFLUAR, Oil, DIC)
  • 63X 1.40 NA (Plan-ApoCHROMAT, Oil, DIC)
  • 25X – Available upon request
  • 40X Water Immersion – Available upon request
  • 63X Water Immersion – Available upon request
  • 100X Oil – Available upon request
  • Argon (458/488/514 nm; 25 mW)
  • Green HeNe (543nm; 1 mW)
  • Red HeNe (633nm; 5 mW)
  • Coherent MIRA/5W VERDI fs TiS for multiphoton excitation imaging

 

Non-descanned Detection Single Channel for MP detection
Z-stepper Motor
Physiology Software
LSM Flexible Scan Package
3D Image Analysis
Becker-Hickl SPC-730 FLIM detector (NDD) and analysis software
LSM Technology objective inverter
Programmable X-Y stage

 

 

System 4 Nikon N-Storm Super Resolution Microscope STRF Room 252

 

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
 Nikon N-Storm Laser SOP

 

Equipped with Elipse Ti inverted TIRF microscope, and two high resolution CCD cameras: Photometrics Cool SNAP HQ2 and Andor iXon3 EMCCD.

 

Objectives: Lasers:
  • CFI PLAN APO 10X NA 0.45
  • CFI Plan APO 20X NA 0.75
  • CFI Super Fluor 40X Oil NA 1.3
  • CFI APO 100X Oil TIRF NA 1.49
  • Violet diode laser: 405 nm
  • Green diode laser: 561 nm
  • Argon laser: 457, 477, 488, and 514 nm
  • Red fiber laser: 647 nm

 

 

System 5 Nikon Swept Field Confocal Microscope with Photoactivation STRF Room 252

 

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
 Nikon Swept Field Confocal SOP

 

Equipped with Nikon Eclipse Ti inverted microscope and a Quant EM 512SC CCD camera. The slit and multi-pinhole scanning allows for rapid imaging.

 

Objectives: Lasers:
  • CFI Plan Apo Lambda 10X NA 0.45
  • CFI Plan APO VC 20X NA 0.75
  • CFI Plan Fluor 40X Oil NA1.30
  • CFI Plan APO VC 60X Oil NA 1.4
  • CFI Plan APO VC 100X Oil NA 1.4
  • Violet diode laser 405 nm
  • Blue diode laser 488 nm
  • Green diode laser 561 nm
  • Red diode laser 640 nm

 

 

System 6 Prairie Confocal/Two-Photon System STRF Room 252

 

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
 Prairie Confocal SOP

 

Equipped with Nikon Eclipse SN1 upright microscope and acoustic optical deflection (AOD) mode, which allows for video rate two-photon imaging.

 

Objectives: Lasers:
  • 25X APO WI NA 1.10 (high NA and LWD)
  • Violet diode laser 405 nm
  • Blue diode laser 488 nm
  • Green diode laser 542 nm
  • Green diode laser 561 nm
  • Red diode laser 640 nm
  • Coherent Chameleon Ti:Sapphire laser with a tunable range 680-1080 nm

 

Widefield Microscopy
System 1 Olympus IX 83 Room STRF 252

 

Objectives:
  • 4X NA 0.16 (UPlansapo)
  • 10X NA 0.33 (UPlan FL)
  • 20X NA 0.70 (Ph2, UCplan FL)
  • 60X NA 1.3 Silicone (UPlansapo)

 

Fully motorized inverted microscope system with ORCA-Flash 4.0 CMOS camera, Prior ProscanIII motorized stage, and LED excitation source.

 

System 2 Nikon Eclipse 2000 Room 2.518U

 

Objectives:
  • 10X 0.45 NA (PlanApo, DIC)
  • 20X 0.75 NA (PlanApo, DIC)
  • 40X 1.30 NA (S Fluor, Oil)
  • 60X (PlanApo TIRF, Oil, DIC)

 

Photometric CoolSnap HQ2 Camera
NIS Elements Software
FURA with Ratiometric Analysis
Fluorescent filters (DAPI, FITC/GFP, Rhodamine, CY5) and DIC

 

 

Fluorescence Stereo Microscopy
System 1 Leica MZ16 FA Fluorescence Stereomicroscope Room 2.664U 16:1 zoom,
Basic magnification
Range 7.1x to 115x
FluoCombi attachment

 

Objectives:
  • 0.5X
  • 1.0X
  • 4X-10x

 

Fluorescence Filters: CFP, GFP, YFP, DsRed
Transmitted, Fluorescence, Goose-neck light sources
Image-ProPlus Analytical Imaging Software
3D-Topography Analysis Software
In-Focus Automation Software
Hamamatsu Orca-AG camera
CRI LCD Filter
Chromophore Imaging

 

 

Whole Animal Imaging (Bioluminescence, Fluorescence)
System 1 IVIS Spectrum Room 1.389U (LAR)
Fluorescence Filters Excitation – 10 (415 nm -760 nm (30 nm bandwidth each))
Emission – 18 (490 nm -850 nm (20 nm bandwidth each))
Quantum Efficiency >85% 500 – 700 nm
>30% 400 – 900nm
Lens f/1 – f/8
Field of View (FOV) Minimum FOV – 3.9 x 3.9 cm
Maximum FOV – 23 x 23 cm (up to 5 mice)
Minimum Detectable Radiance 70 photons/s/cm2/sr
Stage Temperature 20 – 40°C
Transillumination Stage
Isoflurane Gas Anesthesia external chamber, internal nose cones
Dimensions Imaging Chamber Interior Size: 43Wx50Dx60H cm (17 x 20 x 24 in)
Plate reader insert

 

Ancillary Equipment
Isofluorane anesthetizing machine
Mini-vent ventilator
Mouse Ox dosimeter
CO2 Incubator
Bioptechs stage incubator chambers
Imaging system diagnostic equipment
LSM Technologies Objective Inverters
Heater stages
Stage Incubator
Sinks
Small workbench