{"id":704,"date":"2023-04-18T15:15:33","date_gmt":"2023-04-18T20:15:33","guid":{"rendered":"https:\/\/wp.uthscsa.edu\/flow-cytometry\/?page_id=704"},"modified":"2023-07-13T14:53:33","modified_gmt":"2023-07-13T19:53:33","slug":"bd-lsrii","status":"publish","type":"page","link":"https:\/\/wp.uthscsa.edu\/flow-cytometry\/equipment-applications\/bd-lsrii\/","title":{"rendered":"BD LSRII"},"content":{"rendered":"

LSR II Configuration<\/h2>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
LSR II<\/th><\/td><\/td><\/td>\n<\/tr>\n<\/thead>\n
Laser<\/td>Fluorescent Channel<\/td>LSR II Channel Name<\/td>Commonly Used Fluorescent Dyes<\/td>\n<\/tr>\n
405nm Violet<\/td>450\/50<\/td>BV421<\/td>BV421, SuperBright 436, StarBright Violet 440, CF405S, V450, VioBlue, eFluor 450, Pacific Blue, Alexa Fluor 405, Spark Violet 423, Zombie Violet, DAPI,<\/td>\n<\/tr>\n
<\/td>525\/50 505 LP<\/td>BV510<\/td>StarBright Violet 515, StarBright Violet 475, BV 480, BV 510, Pacific Green, Pacific Orange, Zombie Aqua,<\/td>\n<\/tr>\n
<\/td>660\/20 630 LP<\/td>BV650<\/td>StarbBright 670, BV 650, SuperBright 645, Qdot 655<\/td>\n<\/tr>\n
488nm Blue<\/td>525\/50 505 LP<\/td>FITC<\/td>FITC, Alexa Fluor 488, Brilliant Blue 515, Spark Blue 574, Vio515, Zombie Green<\/td>\n<\/tr>\n
<\/td>695\/40 685 LP<\/td>PerCP<\/td>StarBright Blue 700, Brilliant Blue 700, PerCP-eFluor 700, PerCP-Vio 700, PE-Fire 700, PerCP-Cy 5.5, PE-Cy5.5, PerCP<\/td>\n<\/tr>\n
561nm Yellow<\/td>585\/15<\/td>PE<\/td>PE, CF555, Alexa Fluor 555, Spark YG 581<\/td>\n<\/tr>\n
<\/td>610\/20 595 LP<\/td>PE-Texas Red<\/td>PE-CF594, PE-Dazzle 594, PE-Texas Red, ECD, PE-eFluor 610, Propidium Iodide, Zombie Red<\/td>\n<\/tr>\n
<\/td>660\/20 630 LP<\/td>PE-Cy5<\/td>PE-Cy5, PE-Alexa 647, 7-AAD<\/td>\n<\/tr>\n
<\/td>780\/60 750 LP<\/td>PE-Cy7<\/td>PE-Cy7, PE-Vio770,PE-Fire 840<\/td>\n<\/tr>\n
640nm Red<\/td>670\/30<\/td>APC<\/td>APC. Alexa Fluor 647, eFluor 660, APC-Cy5.5, Sytox Red, Ghost Dye Red 710, Live\/Dead Fix Far Red<\/td>\n<\/tr>\n
<\/td>780\/60 750 LP<\/td>APC-Cy7<\/td>APC-Alexa 750, APC-eFluor 780, APC-Vio770, APC-Fire 750, APC-Fire 810, APC-Cy7, Live\/Dead Fix Near IR, Zombie NIR, Ghost Dye Red 780<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"LSR II Configuration LSR II LaserFluorescent ChannelLSR II Channel NameCommonly Used Fluorescent Dyes 405nm Violet450\/50BV421BV421, SuperBright 436, StarBright Violet 440, CF405S, V450, VioBlue, eFluor 450, Pacific Blue, Alexa Fluor 405, Spark Violet 423, Zombie Violet, DAPI, 525\/50 505 LPBV510StarBright Violet 515, StarBright Violet 475, BV 480, BV 510, Pacific Green, Pacific Orange, Zombie Aqua, 660\/20 [...]","protected":false},"author":354,"featured_media":0,"parent":362,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-704","page","type-page","status-publish","hentry"],"yoast_head":"\nBD LSRII - Flow Cytometry<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/wp.uthscsa.edu\/flow-cytometry\/equipment-applications\/bd-lsrii\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"BD LSRII - 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