| Laser | Fluorescent Channel | CytoFlex SRT Channel | Commonly Used Fluorescent Dyes |
| 405 nm | 450/45 BP | PB450 | BV421, SuperBright 436, StarBright Violet 440, CF405S, V450, eFluor 450, Pacific Blue, Alexa Fluor 405, Spark Violet 423, Zombie Violet, DAPI |
| 525/40 BP | KO525 | StarBright Violet 515, StarBright Violet 475, BV 480, BV 510, Pacific Green, Pacific Orange, Zombie Aqua, |
| 610/20 BP | Violet 610 | BV605, Qdot 605 |
| 660/10 BP | Violet 660 | StarbBright 670, BV 650, SuperBright 645, Qdot 655 |
| 780/60 BP | V780 | BV785, BV786 |
| 488 nm | 525/40 BP | FITC | FITC, Alexa Fluor 488, Brilliant Blue 515, Spark Blue 574, Vio515, Zombie Green |
| 690/50 BP | PerCP | StarBright Blue 700, Brilliant Blue 700, PerCP-eFluor 700, PerCP-Vio700, PE-Fire 700, PerCP-Cy5.5, PE-Cy5.5, PerCP |
| 561 nm | 585/42 BP | PE | PE, CF555, Alexa Fluor 555, Spark YG 581 |
| 610/20 BP | ECD | PE-CF594, PE-Dazzle 594, PE-Texas Red, ECD, PE-eFluor 610, Propidium Iodide, Zombie Red |
| 675/30 BP | PC5 | PE-Cy5, PE-Alexa 647, 7-AAD |
| 710/50 BP | PC5.5 | PE-Cy5.5 |
| 780/60 BP | PC7 | PE-Cy7, PE-Vio770, PE-Fire 840 |
| 633 nm | 660/10 BP | APC | APC, Alexa Fluor 647, eFluor 660, APC-Cy5.5, Sytox Red, Ghost Dye Red 710, Live/Dead Fix Far Red |
| 712/25 BP | APC-A 700 | APC-Alexa Fluor 700 |
| 780/60 BP | APC-A 750 | APC-Alexa 750, APC-eFluor 780, APC-Vio770, APC-Fire 750, APC-Fire 810, APC-Cy7, Live/Dead Fix Near IR, Zombie NIR, Ghost Dye Red 780 |
