Publications

2024

Nayak D, Lv D, Yuan Y, Zhang P, Hu W, Nayak A, Ruben EA, Lv Z, Sung P, Hromas R, Zheng G, Zhou D, Olsen SK. (2024). Development and crystal structures of a potent second-generation dual degrader of BCL-2 and BCL-xL. Nat Commun. 15, 2743. PDB: 8FY0, 8FY1, 8FY2

Suating P, Kimberly LB, Ewe MB, Chang SL, Fontenot JM, Sultane PR, Bielawski CW, Decato DA, Berryman OB, Taylor AB, Urbach AR. (2024). Cucurbit[8]uril Binds Nonterminal Dipeptide Sites with High Affinity and Induces a Type II β-Turn. J Am Chem Soc. 146, 7649–7657. CCDC: 2312293.

2023

Jaiswal AS, Dutta A, Srinivasan G, Yuan Y, Zhou D, Shaheen M, Sadideen DT, Kirby A, Williamson EA, Gupta YK, Olsen SK, Xu M, Loranc E, Mukhopadhyay P, Pertsemlidis A, Bishop AJR, Sung P, Nickoloff JA, Hromas R. (2023). TATDN2 resolution of R-loops is required for survival of BRCA1-mutant cancer cells. Nucleic Acids Res. 51, 12224-12241.

Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PDS, Cano KE, Nayak A, Zhao XR, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK. (2023). Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. Nat. Commun. 14, 4786. PDB: 8SE9, 8SEA, 8SEB, 8SV8. Nature Structural & Molecular Biology News and Views Article, February 6, 2024.

Rawal Y, Jia L, Meir A, Zhou S, Kaur H, Ruben EA, Kwon Y, Bernstein KA, Jasin M, Taylor AB, Burma S, Hromas R, Mazin AV, Zhao W, Zhou D, Wasmuth EV, Greene EC, Sung P, Olsen SK. (2023). Structural insights into BCDX2 complex function in homologous recombination. Nature. 619, 640–649. PDB: 8FAZ, 8GBJ

Alwan SN, Taylor AB, Rhodes J, Tidwell M, McHardy SF, LoVerde PT. (2023). Oxamniquine derivatives overcome Praziquantel treatment limitations for Schistosomiasis. PLOS Pathogens. 19, e1011018. PDB: 8E5Q, 8E5R

Clark NE, Katolik A, Gallant P, Welch A, Murphy E, Buerer L, Schorl C, Naik N, Naik MT, Holloway SP, Cano K, Weintraub ST, Howard KM, Hart PJ, Jogl G, Damha MJ, Fairbrother WG. (2023). Activation of human RNA lariat debranching enzyme Dbr1 by binding protein TTDN1 occurs though an intrinsically disordered C-terminal domain. J Biol Chem. 299, 105100.

Amambua-Ngwa A, Button-Simons KA, Li X, Kumar S, Brenneman KV, Ferrari M, Checkley LA, Haile MT, Shoue DA, McDew-White M, Tindall SM, Reyes A, Delgado E, Dalhoff H, Larbalestier JK, Amato R, Pearson RD, Taylor AB, Nosten FH, D’Alessandro U, Kwiatkowski D, Cheeseman IH, Kappe SHI, Avery SV, Conway DJ, Vaughan AM, Ferdig MT, Anderson TJC. (2023). Chloroquine resistance evolution in Plasmodium falciparum is mediated by the putative amino acid transporter AAT1. Nat. Microbiol. 8, 1213-1226.

2022

Herkules F, Yu CH, Taylor AB, Dougherty V, Weintraub ST, Ivanov DN. (2022). Structural and functional asymmetry of RING trimerization controls priming and extension events in TRIM5α autoubiquitylation. Nat. Commun. 13, 7104.

Yuan L, Gao F, Lv Z, Nayak D, Nayak A, Santos Bury PD, Cano KE, Jia L, Oleinik N, Atilgan FC, Ogretmen B, Williams KM, Davies C, El Oualid F, Wasmuth EV, Olsen SK. (2022). Crystal structures reveal catalytic and regulatory mechanisms of the dual-specificity ubiquitin/FAT10 E1 enzyme Uba6. Nat. Commun. 13, 4880. PDB: 7SOL

Lv Z, Cano KE, Jia L, Drag M, Huang TT, Olsen SK. (2022). Targeting SARS-CoV-2 Proteases for COVID-19 Antiviral Development. Front Chem. 9, 819165.

Clark NE, Katolik A, Welch A, Schorl C, Holloway SP, Schuermann JP, Hart PJ, Taylor AB, Damha MJ, Fairbrother WG. (2022). Crystal Structure of the RNA Lariat Debranching Enzyme Dbr1 with Hydrolyzed Phosphorothioate RNA Product. Biochemistry. 61, 2933-2939. PDB: 8DZK

Guzman MA, Rugel A, Alwan SN, Tarpley R, Taylor AB, Chevalier FD, Wendt GR, Collins JJ III, Anderson TJC, McHardy SF, LoVerde PT. (2022). Schistosome Sulfotransferases: Mode of Action, Expression and Localization. Pharmaceutics. 14, 1416.

2021

Patchett S, Lv Z, Rut W, Békés M, Drag M, Olsen SK, Huang TT. (2021). A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease. Cell Rep. 36, 109754. PDB: 7LFU, 7LFV

Yuan L, Lv Z, Adams MJ, Olsen SK. (2021). Crystal structures of an E1-E2-ubiquitin thioester mimetic reveal molecular mechanisms of transthioesterification. Nat. Commun. 12, 2370. PDB: 7K5J

LoVerde PT, Alwan SN, Taylor AB, Rhodes J, Chevalier FD, Anderson TJ, McHardy SF. (2021). Rational approach to drug discovery for human schistosomiasis. Int. J. Parasitol. Drugs Drug Resist. 16, 140-147.

Sea KW, Taylor AB, Thomas ST, Liba A, Bergman IB, Holloway SP, Cao X, Gralla EB, Valentine JS, Hart PJ, Galaleldeen A. (2021). A pH Switch Controls Zinc Binding in Tomato Copper-Zinc Superoxide Dismutase. Biochemistry. 60, 1597-1608. PDB: 3HOG, 3KM1, 3KM2, 3MKG, 3PU7, 3S0P

Yu CH, Bhattacharya A, Persaud M, Taylor AB, Wang Z, Bulnes-Ramos A, Xu J, Selyutina A, Martinez-Lopez A, Cano K, Demeler B, Kim B, Hardies SC, Diaz-Griffero F, Ivanov DN. (2021). Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification. Nat. Commun. 12, 731. PDB: 6U6X, 6U6Y, 6U6Z

Johnson CN, Libich DS. (2021) Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins. J. Vis. Exp. 175, 10.3791/63057.

Baudin A, Moreno-Romero AK, Xu X, Selig EE, Penalva LOF, Libich DS. (2021) Structural Characterization of the RNA-Binding Protein SERBP1 Reveals Intrinsic Disorder and Atypical RNA Binding Modes. Mol. Biosci.  8, 744707.

Baudin A, Xu X, Libich DS. (2021) The 1H, 15N and 13C resonance assignments of the C-terminal domain of Serpine mRNA binding protein 1 (SERBP1). Biomol. NMR Assign. 15, 461-466

2020

Guzman MA, Rugel A, Tarpley RS, Alwan SN, Chevalier FD, Kovalskyy D, Cao X, Holloway SP, Anderson TJC, Taylor AB, McHardy SF and LoVerde PT. An iterative process produces oxamniquine derivatives that kill all human schistosomes. (2020). PLOS Negl. Trop. Dis. 14, e0008517.

Rugel A, Guzman MA, Taylor AB, Chevalier FD, Tarpley RS, McHardy SF, Cao X, Holloway SP, Anderson TJC, Hart PJ and LoVerde PT. (2020). Why does oxamniquine kill Schistosoma mansoni and not S. haematobium and S. japonicum? Int. J. Parasitol. Drugs Drug Resist. 13, 8-15. PDB: 6B4X, 6B4Y, 6B4Z, 6B50, 6B51, 6B52, 6B53, 6B54

Benavides BS, Valandro S, Taylor AB, Cioloboc D, Schanze KS, Kurtz Jr, DM. (2020). Structure of a Zinc Porphyrin-substituted Bacterioferritin and Photophysical Properties of Iron Reduction. Biochemistry. 59, 1618-1629. PDB: 6P8K, 6P8L

Guzman MA, Rugel A, Tarpley RS, Cao X, McHardy SF, LoVerde PT and Taylor AB. (2020). Molecular Basis for Hycanthone Drug Action in Schistosome Parasites. Mol. Biochem. Parasitol. 236, 111257. PDB: 6UUX, 6UUY

Wheeler R., Yu X., Hou C., Mitra P., Chen J.-M., Herkules F., Ivanov D.N., Tsodikov O.V., Rohr J. (2020) Discovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis. Angew. Chem. Int. Ed. Engl. 59, 826-832

2019

Chevalier FD, Le Clec’h W, McDew-White M, Menon V, Guzman M, Holloway SP, Cao X, Taylor AB, Kinungi S, Gouvras A, Webster B, Webster J, Emery AM, Rollinson D, Al Yafae S, Idris MA, Moné H, Mouahid G, Hart PJ, LoVerde PT and Anderson JC. (2019). Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment. PLOS Pathogens. 15, e1007881.

Sherwood LJ, Taylor AB, Hart PJ and Hayhurst A. (2019). Paratope duality and gullying are among the atypical recognition mechanisms employed by a trio of nanobodies to differentiate ebolavirus nucleoproteins. J. Mol. Biol. 431, 4848-4867. PDB: 6U50, 6U51, 6U52, 6U53, 6U54, 6U55

Kim SK, Whitley MJ, Krzysiak TC, Hinck CS, Taylor AB, Zwieb C, Byeon C-H, Zhou X, Mendoza V, López-Casillas F, Furey W, Hinck AP. (2019). Structural adaptation in its orphan domain engenders betaglycan with an alternate mode of growth factor binding relative to endoglin. Structure. 27, 1427-1442. PDB: 6MZN, 6MZP

Balasubramanian S, Pandranki L, Maupin S, Ramasamy K, Taylor AB, Hart PJ, Baseman JB, Kannan TR. (2019). Disulfide bond of Mycoplasma pneumoniae community acquired respiratory distress syndrome toxin is essential to maintain the ADP-ribosylating and vacuolating activities. Cell Microbiol. 21, e13032.

Archer CR, Enslow BT, Taylor AB, De la Rosa V, Bhattacharya A, Shapiro MS. A mutually induced conformational fit underlies Ca2+-directed interactions between calmodulin and the proximal C terminus of KCNQ4 K+ channels. J. Biol. Chem. 294, 6094-6112. PDB: 6N5W

Henen MA, Mahlawat P, Zwieb C, Kodali RB, Hinck CS, Hanna RD, Krzysiak TC, Ilangovan U, Cano KE, Hinck G, Vonberg M, McCabe M, Hinck AP. (2019) TGF-β2 uses the concave surface of its extended finger region to bind betaglycan’s ZP domain via three residues specific to TGF-β and inhibin-α. J. Biol. Chem. 294, 3065-3080

2018

Rugel A, Tarpley RS, Lopez A, Menard T, Guzman MA, Taylor AB, Cao X, Kovalskyy D, Chevalier F, Anderson TJ, Hart PJ, LoVerde PT, McHardy SF. (2018). Design, Synthesis, and Characterization of Novel Small Molecules as Broad Range Anti-Schistosomal Agents. ACS Med. Chem. Lett. 9, 967-973. PDB: 6BDP, 6BDQ, 6BDR, 6BDS, 6MFE

Katti S, Her B, Srivastava AK, Taylor AB, Lockless SW, Igumenova TI. (2018). High affinity interactions of Pb2+ with synaptotagmin I. Metallomics. 10, 1211-1222. PDB: 5VFE, 5VFF, 5VFG

Zhang SY, Clark NE, Freije CA, Pauwels E, Taggart AJ, Okada S, Mandel H, Garcia P, Ciancanelli MJ, Biran A, Lafaille FG, Tsumura M, Cobat A, Luo J, Volpi S, Zimmer B, Sakata S, Dinis A, Ohara O, Garcia Reino EJ, Dobbs K, Hasek M, Holloway SP, McCammon K, Hussong SA, DeRosa N, Van Skike CE, Katolik A, Lorenzo L, Hyodo M, Faria E, Halwani R, Fukuhara R, Smith GA, Galvan V, Damha MJ, Al-Muhsen S, Itan Y, Boeke JD, Notarangelo LD, Studer L, Kobayashi M, Diogo L, Fairbrother WG, Abel L, Rosenberg BR, Hart PJ, Etzioni A, Casanova JL. (2018). Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection. Cell. 172, 952-965.

Ramasamy K, Balasubramanian S, Manickam K, Pandranki L, Taylor AB, Hart PJ, Baseman JB, Kannan TR. (2018). Mycoplasma pneumoniae Community-acquired Respiratory Distress Syndrome Toxin Uses a Novel KELED Sequence for Retrograde Transport and Subsequent Cytotoxicity. MBio. 9, e01663-17.

Yi C, Li G, Ivanov DN, Wang Z, Velasco MX, Hernández G, Kaundal S, Villarreal J, Gupta YK, Qiao M, Hubert CG, Hart MJ, Penalva LOF. (2018) Luteolin inhibits Musashi1 binding to RNA and disrupts cancer phenotypes in glioblastoma cells. RNA Biol. 15, 1420-1432.

Wang Z, Bhattacharya A, White T, Buffone C, McCabe A, Nguyen LA, Shepard CN, Pardo S, Kim B, Weintraub ST, Demeler B, Diaz-Griffero F, Ivanov DN. (2018) Functionality of Redox-Active Cysteines Is Required for Restriction of Retroviral Replication by SAMHD1. Cell Rep. 24, 815-823.

Narasimhan R, Coras R, Rosenthal SB, Sweeney SR, Lodi A, Tiziani S, Boyle D, Kavanaugh A, Guma M. (2018) Serum metabolomic profiling predicts synovial gene expression in rheumatoid arthritis. Arthritis Res. Ther. 20, 164.

Brenner A, Zuniga R, Sun JD, Floyd J, Hart CP, Kroll S, Fichtel L, Cavazos D, Caflisch L, Gruslova A, Huang S, Liu Y, Lodi A, Tiziani S. (2018) Hypoxia Activated Evophosphamide for Treatment of Recurrent Bevacizumab-Refractory Glioblastoma: A phase I surgical study. Neuro. Oncol. 20, 1231-1239.

Bryant JD, Sweeney SR, Sentandreu E, Shin M, Ipas H, Xhemalce B, Momb J, Tiziani S, Appling DR. (2018) Deletion of the neural tube defect-associated gene Mthfd1l disrupts one-carbon and central energy metabolism in mouse embryos. J Biol. Chem. 293, 5821-5833.

2017

Garza JA, Taylor AB, Sherwood LJ, Hart PJ, Hayhurst A. (2017). Unveiling a drift resistant cryptotope within Marburgvirus nucleoprotein recognized by llama single domain antibodies. Front. Immunol. 8, 1234. PDB: 4W2O, 4W2P, 4W2Q, 6APO, 6APP, 6APQ

Choi JY, Fuerst R, Knapinska AM, Taylor AB, Smith L, Cao X, Hart PJ, Fields GB, Roush WR. (2017). Structure-Based Design and Synthesis of Potent and Selective Matrix Metalloproteinase 13 Inhibitors. J. Med. Chem. 60, 5816-5825. PDB: 5UWK, 5UWL, 5UWM, 5UWN

Fetherolf MM, Boyd SD, Taylor AB, Blackburn NJ, Hart PJ, Winge DR, Winkler DD. (2017). Copper-Zinc Superoxide Dismutase is Activated through a Sulfenic Acid Intermediate at a Copper-ion Entry Site. J. Biol.Chem. 292, 12025-12040. PDB: 5U9M

Taylor AB, Roberts KM, Cao X, Clark NE, Holloway SP, Donati E, Polcaro CM, Pica-Mattoccia L, Tarpley RS, McHardy SF, Cioli D, LoVerde PT, Fitzpatrick PF, Hart PJ. (2017). Structural and Enzymatic Insights into Species-specific Resistance to Schistosome Parasite Drug Therapy. J. Biol. Chem. 292, 11154-1164. PDB: 5TIV, 5TIW, 5TIX, 5TIY, 5TIZ

Katti S, Nyenhuis SB, Her B, Srivastava AK, Taylor AB, Hart PJ, Cafiso DS, Igumenova TI. (2017). Non-native metal ion reveals the role of electrostatics in Synaptotagmin 1-membrane interactions. Biochemistry. 56, 3283-3295. PDB: 5T0R, 5T0S

Kim SK, Myers L, Hinck CS, Cano K, Thirangala A, Iskra B, Brothers M, Vonberg M, Leal B, Richter B, Kodali R, Taylor AB, Du S, Barnes C, Calero G, Hart PJ, Hart MJ, Demeler B, Hinck AP. (2017). An Engineered TGF-beta Monomer that Functions as a Dominant Negative to Block TGF-beta Signaling. J. Biol. Chem. 292, 7173-7188. PDB: 5TX2, 5TX4, 5TX6 Editor’s Pick

Katolik A, Clark NE, Tago N, Montemayor EJ, Hart PJ, Damha MJ. (2017). Fluorescent Branched RNAs for High-throughput Analysis of Dbr1 Enzyme Kinetics and Inhibition. ACS Chem Biol. 12, 622-627.

2016

Clark NE, Katolik A, Roberts KM, Taylor AB, Holloway SP, Schuermann JP, Montemayor EJ, Stevens SW, Fitzpatrick PF, Damha MJ, Hart PJ. (2016). Metal dependence and branched RNA cocrystal structures of the RNA lariat debranching enzyme Dbr1. Proc. Natl. Acad. Sci. 113, 14727-14732. PDB: 5K71, 5K73, 5K77, 5K78

Ayers JI, Diamond J, Sari A, Fromholt S, Galaleldeen A, Ostrow LW, Glass JD, Hart PJ, Borchelt DR. (2016). Distinct conformers of transmissible misfolded SOD1 distinguish human SOD1-FALS from other forms of familial and sporadic ALS. Acta Neuropathol. 132, 827-840.

Wong SJ, Gearhart MD, Taylor AB, Nanyes DR, Ha DJ, Robinson AK, Artigas JA, Lee OJ, Demeler B, Hart PJ, Bardwell VJ, Kim CA. (2016). KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1. Structure. 24, 1795-1801. PDB: 5JH5

Peterson RL, Galaleldeen A, Villarreal J, Taylor AB, Cabelli DE, Hart PJ, Culotta VC. (2016). The Phylogeny and Active Site Design of Eukaryotic Cu-only Superoxide Dismutases. J. Biol. Chem. 291, 20911-20923. PDB: 5KBK, 5KBL, 5KBM

Chevalier FD, Le Clec’h W, Eng N, Rugel AR, de Assis RR, Oliveira G, Holloway SP, Cao X, Hart PJ, LoVerde PT, Anderson TJ. (2016). Independent origins of loss-of-function mutations conferring oxamniquine resistance in a Brazilian schistosome population. Int. J. Parisitol. 46, 417-424.

Meisburger SP, Taylor AB, Khan CA, Zhang S, Fitzpatrick PF, Ando N. (2016). Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-ray Scattering. J. Am. Chem. Soc. 138, 6506-6516. PDB: 5EGQ, 5FGJ

Wu X, Kim H, Seravalli J, Barycki JJ, Hart PJ, Gohara DW, Di Cera E, Jung WH, Kosman DJ, Lee J. (2016). Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to Apo-Fet3p Multi-Copper Ferroxidase. J. Biol. Chem. 291, 9796-9806.

2015

Taylor AB, Pica-Mattoccia L, Polcaro CM, Donati E, Cao X, Basso A, Guidi A, Rugel AR, Holloway SP, Anderson TJ, Hart PJ, Cioli D, and LoVerde PT. (2015). Structural and functional characterization of the enantiomers of the antischistosomal drug oxamniquine. PloS Negl. Trop. Dis. 9, e0004132. PDB: 5BYJ, 5BYK

Tago N, Katolik A, Clark NE, Montemayor EJ, Seio K, Sekine M, Hart PJ, and Dahma MJ. (2015). Design, Synthesis, and Properties of Phosphoramidate 2′,5′-Linked Branched RNA: Toward the Rational Design of Inhibitors of the RNA Lariat Debranching Enzyme. J. Org. Chem. 80, 10108-10118.

Yudina Z, Roa A, Johnson R, Biris N, de Souza Aranha Vieira A, Tsiperson V, Reszka N, Taylor AB, Hart PJ, Demeler B, Diaz-Griffero F, and Ivanov DN. (2015). RING dimerization links higher-order assembly of TRIM5α to synthesis of K63-linked polyubiquitin. Cell Reports. 12, 788-797. PDB: 4TKP

Becker A, Kannan TR, Taylor AB, Pakhomova ON, Zhang Y, Somarajan SR, Galaleldeen A, Holloway SP, Baseman JB, and Hart PJ. (2015). Structure of CARDS toxin, a unique ADP-ribosylating and vacuolating cytotoxin from Mycoplasma pneumoniae. Proc. Natl. Acad. Sci. 112, 5165-5170. PDB: 4TLV, 4TLW

Sea K, Sohn SH, Durazo A, Sheng Y, Shaw BF, Cao X, Taylor AB, Whitson LJ, Holloway SP, Hart PJ, Cabelli DE, Gralla EB, Valentine JS. (2015). Insights into the Role of the Unusual Disulfide Bond in Copper-Zinc Superoxide Dismutase. J. Biol. Chem. 290, 2405-2418. PDB: 4MCM, 4MCN

2014

Bhattacharya A, Alam SL, Fricke T, Zadrozny K, Sedzicki J, Taylor AB, Demeler B, Pornillos O, Ganser-Pornillos BK, Diaz-Griffero F, Ivanov DN, Yeager M. (2014). Structural basis of HIV-1 capsid recognition by PF74 and CPSF6. Proc. Natl. Acad. Sci. 111, 18625-18630. PDB: 4WYM

Spicer TP, Jiang J, Taylor AB, Choi JY, Hart PJ, Roush WR, Fields GB, Hodder PS, Minond D.(2014). Characterization of Selective Exosite-Binding Inhibitors of Matrix Metalloproteinase 13 That Prevent Articular Cartilage Degradation In Vitro. J. Med. Chem. 57, 9598-9611. PDB: 4L19

Montemayor EJ, Katolik A, Clark NE, Taylor AB, Schuermann JP, Combs DJ, Johnsson R, Holloway SP, Stevens SW, Damha MJ, Hart PJ. (2014). Structural basis of lariat RNA recognition by the intron debranching enzyme Dbr1. Nucleic Acids Res. 42, 10845-10855. PDB: 4PEF, 4PEG, 4PEH, 4PEI, NAR Breakthrough Article.

Nanyes DR, Junco SE, Taylor AB, Robinson AK, Patterson NL, Shivarajpur A, Halloran J, Hale SM, Kaur Y, Hart PJ, Kim CA. (2014). Multiple polymer architectures of human polyhomeotic homolog 3 sterile alpha motif. Proteins. 82, 2823-2830. PDB: 4PZN, 4PZO

Kannan TR, Krishnan M, Ramasamy K, Becker A, Pakhomova ON, Hart PJ, Baseman JB. (2014). Functional mapping of community-acquired respiratory distress syndrome (CARDS) toxin of Mycoplasma pneumoniae defines regions with ADP-ribosyltransferase, vacuolating and receptor-binding activities. Mol. Microbiol. 93, 568-581.

Ayers JI, Xu G, Pletnikova O, Troncoso JC, Hart PJ, Borchelt DR. (2014). Conformational specificity of the C4F6 SOD1 antibody; low frequency of reactivity in sporadic ALS cases. Acta Neuropathol. Commun. 2, 55.

Gleason JE, Galaleldeen, A, Peterson RL, Taylor AB, Holloway SP, Waninger-Saroni J, Cormack BP, Cabelli DE, Hart PJ, Culotta VC. (2014). Candida albicans SOD5 represents the prototype of an unprecedented class of Cu-only superoxide dismutases required for pathogen defense. Proc. Natl. Acad. Sci. 111, 5866-5871. PDB: 4N3T, 4N3U

Katolik A, Johnsson R, Montemayor E, Lackey JG, Hart PJ, Damha MJ. (2014). Regiospecific Solid-phase Synthesis of Branched Oligoribonucleotides that Mimic Intronic Lariat RNA Intermediates. J. Org. Chem. 79, 963-975.

Ivanova MI, Sievers SA, Guenther EL, Johnson LM, Winkler DD, Galaleldeen A, Sawaya MR, Hart PJ, Eisenberg DS. (2014). Aggregation-triggering segments of SOD1 fibril formation support a common pathway for familial and sporadic ALS. Proc. Natl. Acad. Sci. 111, 197-201.

2013

Valentim CL, Cioli D, Chevalier FD, Cao X, Taylor AB, Holloway SP, Pica-Mattoccia L, Guidi A, Basso A, Tsai IJ, Berriman M, Carvalho-Queiroz C, Almeida M, Aguilar H, Frantz DE, Hart PJ, LoVerde PT, Anderson TJ. (2013). Genetic and molecular basis of drug resistance and species-specific drug action in schistosome parasites. Science. 342, 1385-1389. PDB: 4MUA, 4MUB

Morales KA, Yang Y, Long Z, Li P, Taylor AB, Hart PJ, Igumenova TI. (2013). Cd2+ as a Ca2+Surrogate in Protein-membrane Interactions: Isostructural but not Isofunctional. J. Am. Chem. Soc. 135, 12980-12983.PDB: 4L1L

Galaleldeen A, Taylor AB, Chen D, Schuermann JP, Holloway SP, Hou S, Gong S, Zhong G, Hart PJ. (2013). Structure of the Chlamydia trachomatis Immunodominant Antigen Pgp3. J. Biol. Chem. 288, 22068-22079. PDB: 4JDM, 4JDN, 4JDO in ASBMB Today September 2013.

Gaweska, HM, Taylor AB, Hart PJ, Fitzpatrick PF. (2013). The Structure of the Flavoprotein Tryptophan-2-Monooxygenase, a Key Enzyme in the Formation of Galls in Plants. Biochemistry. 52, 2620-2626.PDB: 4IV9

Junco SE, Wang R, Gaipa JC, Taylor AB, Schirf V, Gearhart MD, Bardwell VJ, Demeler B, Hart, PJ, Kim CA. (2013). Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs. Structure. 21, 665-671. PDB: 4HPL, 4HPM

Aguirre JD, Clark HM, McIlvin M, Vazquez C, Palmere SL, Grab D, Seshu J, Hart PJ, Saito M, Culotta VC. (2013). A Manganese-rich Environment Supports Superoxide Dismutase Activity in a Lyme Disease Pathogen, Borrelia burgdorferi. J. Biol. Chem. 288, 8468-8478.

2012

Adachi MS, Taylor AB, Hart PJ, Fitzpatrick PF. (2012). Mechanistic and Structural Analyses of the Roles of Active Site Residues in the Yeast Polyamine Oxidase Fms1: Characterization of the N195A and D94N Enzymes. Biochemistry. 51, 8690-8697. PDB: 4GDP

Fang H, Caranto JD, Mendoza R, Taylor AB, Hart PJ, Kurtz Jr DM.(2012). Histidine ligand variants of a flavo-diiron protein: Effects on structure and activities. J. Biol. Inorg. Chem. 17, 1231-1239. PDB: 4DIK, 4DIL

Biris N, Yang Y, Taylor AB, Tomashevski A, Guo M, Hart PJ, Diaz-Griffero F, Ivanov DN. (2012). Structure of the rhesus monkey TRIM5α PRYSPRY domain, the HIV capsid recognition module. Proc. Natl. Acad. Sci. 109, 13278-13283. PDB: 3UV9, 2LM3 in Protein Data Bank Molecule of the Month July 2013

Adachi MS, Taylor AB, Hart PJ, Fitzpatrick PF. (2012). Mechanistic and Structural Analyses of the Role of His67 in the Yeast Polyamine Oxidase Fms1. Biochemistry. 51, 4888-4897. PDB: 4ECH

Bouldin SD, Darch MA, Hart PJ, Outten CE. (2012). Redox properties of the disulfide bond of human Cu,Zn superoxide dismutase and the effects of human glutaredoxin 1. Biochem J. 446, 59-67.

Leitch JM, Li CX, Baron JA, Matthews LM, Cao X, Hart PJ, Culotta VC. (2012). Post-translational modification of Cu/Zn superoxide dismutase under anaerobic conditions. Biochemistry. 51, 677-685.

2011

Pau VPT, Smith FJ, Taylor AB, Parfenova LV, Samakai E, Callaghan MM, Abarca-Heidemann K, Hart PJ, and Rothberg BS. (2011). Structure and function of Multiple Ca2+-binding sites in a K+ channel RCK domain. Proc. Natl. Acad. Sci. 108, 17684-17689. PDB: 3RBX, 3RBZ

Childers BM, Cao X, Weber GG, Demeler B, Hart PJ, Klose KE. (2011). N-terminal residues of theVibrio cholerae virulence regulatory protein ToxT involved in dimerization and modulation by fatty acids. J. Biol. Chem. 286, 28644-28655.

Chinai JM, Taylor AB, Ryno LM, Hargreaves ND, Morris CA, Hart PJ, and Urbach AR. (2011). Molecular Recognition of Insulin by a Synthetic Receptor. J. Am. Chem. Soc. 133, 8810-8813. PDB: 3Q6E

2010

Chen D, Lei L, Lu C, Galaleldeen A, Hart PJ, and Zhong G. (2010). Characterization of Pgp3, a Chlamydia trachomatis plasmid-encoded immunodominant antigen. J Bacteriol. 192, 6017-6024.

Seetharaman SV, Taylor AB, Holloway S, Hart PJ. (2010). Structures of mouse SOD1 and human/mouse SOD1 chimeras.  Arch. Biochem. Biophys. 503, 183-190. PDB: 3GTT, 3GTV, 3LTV

Wang R, Taylor AB, Leal BZ, Chadwell LV, Ilangova U, Robinson AK, Schirf V, Hart PJ, Lafer EM, Demeler B, Hinck AP, McEwen DG, and Kim CA. (2010). Polycomb Group Targeting Through Different Binding Partners of RING1B C-terminal Domain. Structure. 18, 966-975. PDB: 3GS2, 3IXS

Seetharaman SV, Winkler DD, Taylor AB, Cao X, Whitson LJ, Doucette PA, Valentine JS, Schirf V, Demeler B, Carroll MC, Culotta VC, and Hart PJ. (2010). Disrupted Zinc-binding Sites in Structures of Pathogenic SOD1 Variants D124V and H80R.  Biochemistry. 49, 5714-5725.  PDB: 3H2P, 3H2Q, 3H2R

Tormos JR, Taylor AB, Daubner SC, Hart PJ, and Fitzpatrick PF. (2010).  Identification of a Hypothetical Protein from Podospora anserina as a Nitroalkane Oxidase. Biochemistry. 49, 5035-5031. PDB: 3MKH

Pakhomova ON, Taylor AB, Becker A, Holloway SP, Kannan TR, Baseman JB, and Hart PJ. (2010).  Crystallization of Community-acquired Respiratory Distress Syndrome Toxin from Mycoplasma pneumoniae. Acta Cryst. F66, 294-296.

You Z, Cao X, Taylor AB, Hart PJ, and Levine, RL. (2010).  Characterization of a Covalent Polysulfane Bridge in Copper-Zinc Superoxide Dismutase. Biochemistry. 49, 1191-1198. PDB: 3K91

2009

Galaleldeen A, Strange R, Whitson LJ, Antonyuk S, Narayana N, Taylor AB, Schuermann JP, Holloway SP, Hasnain SS, and Hart PJ.  (2009).  Structural and Biophysical Properties of Metal-Free Pathogenic SOD1 Mutants A4V and G93A.  Arch. Biochem. Biophys.  492, 40-47.  PDB: 3GZO, 3GZP, 3GZQ, 2WKO

Konkle ME, Muellner SK, Schwander AL, Dicus MM, Pokhrel R, Britt RD, Taylor AB, and Hunsicker-Wang LM. (2009).  Effects of pH on the Rieske Protein from Thermus thermophilus: A Spectroscopic and Structural Analysis. Biochemistry. 48, 9848-9857.  PDB: 3FOU

Tiwari A, Liba A, Sohn SH, Seetharaman SV, Bilsel O, Matthews CR, Hart PJ, Valentine JS, and Hayward LJ.  (2009).  Metal deficiency increases aberrant hydrophobicity of mutant superoxide dismutases that cause amyotrophic lateral sclerosis.  J. Biol. Chem.  284, 27746-27758.

Seetharaman SV, Prudencio M, Karch C, Holloway SP, Borchelt DR, and Hart PJ. (2009).  Immature copper-zinc superoxide dismutase and familial amyotrophic lateral sclerosis. Exp. Biol. Med. 234, 1140-1154.

Balasubramanian S, Kannan TR, Hart PJ, and Baseman JB. (2009). Amino acid changes in elongation factor Tu of Mycoplasma pneumoniae and Mycoplasma genitalium influence fibronectin binding. Infect. Immun. 77, 3533-3541.

Leitch JM, Jensen LT, Bouldin SD, Outten CE, Hart PJ, and Culotta VC. (2009). Activation of Cu,Zn-superoxide dismutase in the absence of oxygen and the copper chaperone CCS. J. Biol. Chem. 284, 21863-21871.

Prudencio M, Hart PJ, Borchelt DR, and Andersen PM. (2009). Variation in aggregation propensities among ALS-associated variants of SOD1: correlation to human disease. Hum. Mol. Genet. 18, 3217-3226.

Karch CM, Prudencio M, Winkler DD, Hart PJ, and Borchelt DR. (2009). Role of mutant SOD1 disulfide oxidation and aggregation in the pathogenesis of familial ALS. Proc. Natl. Acad. Sci. 106, 7774-7779.

Chen D, Chai J, Hart PJ, and Zhong G. (2009). Identifying catalytic residues in CPAF, a Chlamydia-secreted protease. Arch. Biochem. Biophys.  485, 16-23.

Winkler DD, Schuermann JP, Cao X, Holloway SP, Borchelt DR, Carroll MC, Proescher JB, Culotta VC, and Hart PJ. (2009). Structural and biophysical properties of the pathogenic SOD1 variant H46R/H48Q. Biochemistry. 48, 3436-3447. PDB: 3GQF

2008

Schuermann JP, Jiang J, Cuellar J, Llorca O, Wang L, Gimenez LE, Jin S, Taylor AB, Demeler B, Morano KA, Hart PJ, Valpuesta JM, Lafer EM, and Sousa, R. (2008).  Structure of the Hsp110:Hsc70 nucleotide exchange machine. Mol. Cell. 31, 232-243. PDB: 3C7N

Cao X, Antonyuk S, Seetharaman SV, Whitson LJ, Taylor AB, Holloway SP, Strange RW, Doucette PA, Valentine JS, Tiwari A, Hayward LJ, Padua S, Cohlberg JA, Hasnain SS, and Hart PJ. (2008). Structures of the G85R variant of SOD1 in Familial ALS. J. Biol. Chem. 283, 16169-16177. PDB: 2VR6, 2VR7, 2VR8, 3CQP, 3CQQ

Taylor AB, Meyer B, Leal BZ, Kötter P, Schirf V, Demeler B, Hart PJ, Entian KD, and Wöhnert, J.  (2008). The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.  Nucleic Acids Res. 36, 1542-1554.  PDB: 3BBD, 3BBE, 3BBH

Taylor AB, Hu G, Hart PJ, and McAlister-Henn L. (2008). Allosteric motions in structures of yeast NAD+-specific isocitrate dehydrogenase.  J. Biol. Chem.  283, 10872-10880.  PDB: 3BLV, 3BLW, 3BLX in Protein Data Bank Molecule of the Month September 2010, October 2012

Groppe J, Hinck CS, Samavarchi-Tehrani P, Zubieta C, Schuermann JP, Taylor AB, Schwarz PM, Wrana JL, and Hinck, AP.  (2008). Cooperative assembly of TGF-β superfamily signaling complexes Is mediated by two disparate mechanisms and distinct modes of receptor binding.  Mol. Cell. 29, 157-168.  PDB: 2PJY

2007

Quintanar L, Stoj C, Taylor AB, Hart PJ, Kosman DJ, and Solomon EI. (2007). Shall we dance? How a multicopper oxidase chooses its electron transfer partner. Acc. Chem. Res. 40, 445-452.

Jiang J, Maes EG, Taylor AB, Wang L, Hinck AP, Lafer EM, and Sousa, R.  (2007). Structural basis of J cochaperone binding and regulation of Hsp70. Mol. Cell.  28, 422-433. PDB: 2QW9, 2QWL, 2QWM, 2QWN, 2QWO, 2QWP, 2QWQ, 2QWR

Hu G, Taylor AB, McAlister-Henn L, and Hart PJ. (2007). Crystal structure of the yeast nicotinamidase Pnc1p.  Arch. Biochem. Biophys.  461, 66-75.  PDB: 2H0R

Galaleldeen A and Hart PJ. (2007) “Human copper-zinc superoxide dismutase and familial amyotrophic lateral sclerosis” in: Protein Reviews: Protein Misfolding, Aggregation and Conformational Diseases. (Atassi, M.Z. ed.). Springer Science + Business Media, LLC, New York, U.S.A., pp. 327-344.

Wang J, Caruano-Yzermans A, Rodriguez A, Schuermann JP, Slunt HH, Cao X, Gitlin J, Hart PJ, and Borchelt DR. (2007). Disease-associated mutations at copper ligand histidine residues of superoxide dismutase 1 diminish the binding of copper and compromise dimer stability.  J. Biol. Chem.  282, 345-352.  PDB: 2NNX

2006

Heitmann LM, Taylor AB, Hart PJ, and Urbach AR.  (2006).  Sequence-specific recognition and cooperative dimerization of N-terminal aromatic peptides in aqueous solution by a synthetic host.  J. Am. Chem. Soc.  128, 12574-12581.

Carroll MC, Outten CE, Proescher JB, Rosenfeld L, Watson WH, Whitson LJ, Hart PJ, Jensen LT, and Culotta VC. (2006). The effects of glutaredoxin and copper activation pathways on the disulfide and stability of Cu,Zn superoxide dismutase.  J. Biol. Chem.  281, 28648-28656.

Hart PJ.  (2006).  Pathogenic superoxide dismutase structure, folding, aggregation and turnover.  Curr. Opin. Chem. Biol. 10, 131-138.

Jiang J, Lafer EM, and Sousa R. (2006). Crystallization of a functionally intact Hsp70 chaperone. Acta Cryst. F62, 39-43.

2005

Taylor AB, Stoj CS, Ziegler L, Kosman DJ, and Hart PJ.  (2005).  The copper-iron connection in biology: Structure of the metallo-oxidase Fet3p.  Proc. Natl. Acad. Sci. 102, 15459-15464.  PDB: 1ZPU

Di Noto L, Whitson LJ, Cao X, Hart PJ, and Levine RL.  (2005).  Proteasomal degradation of mutant superoxide dismutases linked to amyotrophic lateral sclerosis.  J. Biol. Chem. 280, 39907-39913.

Jiang J, Prasad K, Lafer EM, and Sousa, R. (2005) Structural basis of interdomain communication in the Hsc70 chaperone. Mol. Cell. 20, 513-524.  PDB: 1YUW

Whitson LJ and Hart PJ. (2005) “Copper-zinc superoxide dismutase and familial amyotrophic lateral sclerosis.” in: Metal Ions in Biological Systems. Vol 45 (Sigel, A., Sigel, H, and Sigel, R.O.K. eds) John Wiley & Sons Ltd., Chichester, UK.

Hart PJ and Valentine JS. (2005).  “Metal-deficient copper-zinc superoxide dismutase and familial amyotrophic lateral sclerosis” in Medicinal Inorganic Chemistry.  (Sessler, J.L., Doctrow, S.R., McMurray, T.S. and Lippard, S.J. eds).  ACS Books, Washington D.C., U.S.A. pp. 348-365.

Hart PJ, Nersissian AM, and DeBeer GS.  (2005).  “Copper Proteins with Type I Sites” in Encyclopedia of Inorganic Chemistry, Vol. 2. (King, R.B., Atwood, D.A., Crabtree, R.H., Lukehart, C.M. and Scott, R.A. eds.) John Wiley & Sons, Ltd.  Chichester, U.K.

Hu G, Taylor AB, McAlister-Henn L, and Hart PJ. (2005). Crystallization and preliminary X-ray crystallographic analysis of yeast NAD+-specific isocitrate dehydrogenase. Acta Crystallogr. F61, 486-488.

Antonyuk S, Elam JS, Hough MA, Strange RW, Doucette PA, Rodriguez, JA, Hayward LJ, Valentine JS, Hart PJ, and Hasnain SS. (2005).  Structural consequences of the familial amyotrophic lateral sclerosis SOD1 mutant His46Arg. Protein Sci. 14, 1201-1213.

2004

Doucette PA, Whitson LJ, Cao X, Schirf V, Demeler B, Valentine JS, Hansen JC, and Hart PJ.  (2004).  Dissociation of human copper-zinc superoxide dismutase dimers using chaotrope and reductant: Insights into the molecular basis for dimer stability. J. Biol. Chem. 279, 54558-54566.

Laliberte J, Whitson LJ, Beaudoin J, Holloway SP, Hart PJ, and Labbe S.  (2004).  The Schizosaccharomyces pombe Pccs protein functions in both copper trafficking and metal detoxification pathways.  J. Biol. Chem.  279, 28744-28755.

Hough MA, Grossmann JG, Antonyuk SV, Strange RW, Doucette PA, Rodriguez JA, Whitson LJ, Hart PJ, Hayward LJ, Valentine JS, and Hasnain SS. (2004).  Dimer destabilization in superoxide dismutase may result in disease-causing properties: structures of motor neuron disease mutants. Proc. Natl. Acad. Sci. U.S.A. 101, 5976-5981.

2003

Elam JS, Taylor AB, Strange R, Antonyuk S, Doucette PA, Rodriguez JA, Hasnain SS, Hayward LJ, Valentine JS, Yeates TO, and Hart PJ. (2003).  Amyloid-like filaments and water-filled nanotubes formed by SOD1 mutants linked to familial ALS. Nat. Struct. Biol. 10, 461-467.  PDB: 1OEZ, 1OZT, 1OZU

Elam JS, Malek K, Rodriguez JA, Doucette PA, Taylor AB, Hayward LJ, Cabelli DE, Valentine JS, and Hart PJ. (2003).  An alternative mechanism of bicarbonate-mediated peroxidation by copper-zinc superoxide dismutase.  J. Biol. Chem. 278, 21032-21039. PubMed PDB: 1P1V

Taylor AB, Benglis Jr, DM, Dhandayuthapani S, and Hart PJ. (2003).  Structure of Mycobacterium tuberculosis methionine sulfoxide reductase A in complex with protein-bound methionine.  J. Bacteriol. 185, 4119-4126.  PDB: 1NWA

Jiang J, Taylor AB, Prasad K, Ishikawa-Brush Y, Hart PJ, Lafer EM, and Sousa R. (2003). Structure-function analysis of the auxilin J-domain reveals an extended Hsc70 interaction interface. Biochemistry. 42, 5748-53. PDB:1NZ6

Strange R, Antonyuk S, Hough MA, Doucette PA, Rodriguez JA, Hart PJ, Hayward LJ, Valentine JS, and Hasnain SS. (2003).  The structure of holo and metal deficient wild type human Cu,Zn superoxide dismutase and its relevance to familial amyotrophic lateral sclerosis.  J. Mol. Biol. 328, 877-91.  PDB: 1HL4, 1HL5

Valentine JS and Hart PJ. (2003).  Misfolded CuZnSOD and ALS. Proc. Natl. Acad. Sci. U.S.A.100, 3617-3622.

2002

Hart PJ, Deep S, Taylor AB, Shu Z, Hinck CS, and Hinck AP.  (2002). Crystal Structure of the Human TGF-beta Type II Receptor Extracellular Domain in Complex with TGF-beta3. Nat. Struct. Biol. 9, 203-208.  PDB: 1KTZ

Elam JS, Thomas ST, Holloway SP, Taylor AB, and Hart PJ. (2002). Copper Chaperones.  In: Advances in Protein Chemistry. (Valentine, J.S. and Gralla, E.B., eds.) Academic Press, San Diego, CA. pp. 151-219.

Pascal JM, Hart PJ, Hecht NB, and Robertus JD. (2002). Crystal structure of TB-RBP, a novel RNA-binding and regulating protein.  J. Mol. Biol. 319, 1049-1057.  PDB: 1KEY

2001

Nersissian AM, Hart PJ, and Valentine JS. (2001). Stellacyanin, a member of the phytocyanin family of plant proteins. In: Handbook of Metalloproteins (Messerschmidt, A., Huber, R., Poulos, T., and Wieghardt, K., eds) John Wiley and Sons, Ltd.,  Chichester, England. pp. 1219-1234.

2000

Hall LT, Sanchez RJ, Holloway SP, Zhu H, Stine JE, Lyons TJ, Demeler B, Schirf V, Hansen JC, Nersissian AM, Valentine JS, and Hart PJ. (2000). X-ray crystallographic and analytical ultracentrifugation analyses of truncated and full-length yeast copper chaperones for SOD (LYS7): a dimer-dimer model of LYS7-SOD association and copper delivery. Biochemistry. 39, 3611-3623. PDB: 1EJ8

Zhu H, Shipp E, Sanchez RJ, Liba A, Stine JE, Hart PJ, Gralla EB, Nersissian AM, and Valentine JS.  (2000). Cobalt(2+) binding to human and tomato copper chaperone for superoxide dismutase: implications for the metal ion transfer mechanism. Biochemistry. 39, 5413-5421.

1999

Valentine JS, Hart PJ, and Gralla EB. (1999). “Copper-zinc superoxide dismutase and ALS.” In: Advances in Experimental Medicine and Biology: Copper Transport and its Disorders:  Molecular and Cellular Aspects (Leone, A., and Mercer, J.F.B. eds.) Plenum Press, New York, New York. pp. 193-203.